16srRNA Meta-genomics using Next generation Sequencing

16S rRNA gene sequencing is commonly used for identification, classification and quantitation of microbes within complex biological mixtures. V3-V4 region sequencing by NGS of the 16srRNA gene to identify the bacterial diversity in a sample from DNA

18srRNA Meta-genomics using Next generation Sequencing

18S rRNA gene sequencing is commonly used to identify, classify, and quantify microbes within complex biological mixtures. 18S rRNA gene has nine variable regions (V1-V9).

18S rRNA sequences could be used for taxonomic resolutions and diversity analysis in eukaryotic communities.

18S rRNA is mainly used for high resolution taxonomic studies of fungi, while the ITS region is mainly used for fungal diversity studies as a fungal barcode marker

Bacterial whole genome sequencing

Whole-genome sequencing (WGS) is a comprehensive method for analyzing entire genomes. This method is commonly associated with sequencing human genomes, the scalable, flexible nature of next-generation sequencing (NGS) technology makes it equally useful for sequencing any species, such as agriculturally important livestock, plants, or disease-related microbes.

Shotgun Metagenomics

Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism’s genome. The method involves randomly breaking up the genome into small DNA fragments that are sequenced individually.

Sanger Sequencing

(Mutation Studies)

Sanger sequencing is a robust testing strategy able to determine whether a point mutation or small deletion/duplication is present. Sanger sequencing with 99.99% accuracy is the “gold standard” for clinical research sequencing.

RT PCR gene Expression Analysis

Real time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus.